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41.
42.
史氏鲟外周血细胞的显微及超微结构 总被引:3,自引:0,他引:3
采用血细胞计数、光学显微及电子显微技术对二龄史氏鲟外周血细胞的数目、形态及结构进行了研究。二龄史氏鲟红细胞的数目为47.75×104个/mm3,白细胞数目为2.9万个/mm3,其中淋巴细胞所占比率最高。史氏鲟的外周血中除正常红细胞外,还有处于分裂状态及未成熟的红细胞。史氏鲟外周血中的白细胞有四种类型,分别为淋巴细胞、粒细胞、血栓细胞和单核细胞。其中粒细胞有两种,即嗜中性粒细胞和嗜酸性粒细胞。嗜中性粒细胞含有多种形状的核,其中分叶的核数目较多,粒细胞及淋巴细胞均类似于哺乳动物。对史氏鲟外周血细胞细微结构的观察显示:红细胞中具有少量的细胞器;淋巴细胞结构典型;单核细胞较粒细胞稍小且具有较多线粒体;血栓细胞具有梭形和圆形两种,胞质较少,其中梭形的血栓细胞胞质几乎透明;对粒细胞的颗粒按照形状和电子密度进行了分类。 相似文献
43.
西伯利亚鲟(Acipenser baerii)致病性维氏气单胞菌的分离鉴定 总被引:5,自引:0,他引:5
摘要:【目的】本研究旨在寻找引起养殖西伯利亚鲟鱼(Acipenser baerii)病害的致病因子。【方法】从北京地区自然患病的西伯利亚鲟鱼体内分离到致病菌株X-1-06909,采用生理生化鉴定结合16S rRNA基因序列的系统发育学分析确定该菌株的系统发育地位。同时采用琼脂扩散法对抗菌类药物的敏感性进行测定。【结果】菌株X-1-06909与Aeromonas veronii ATCC 35624T的16S rRNA基因序列相似性达99.6%;结合形态特征与生理生化测定结果,革兰氏阴性杆菌,具极生单鞭毛 相似文献
44.
Since first large-scale attempts to culture sturgeon from the larval stage were carried out in the former U.S.S.R. at the end of the nineteenth century, rearing technology has advanced considerably during the last 20 years and noticeable improvements in incubation and larval rearing techniques have been implemented in normal hatchery procedures. Siberian sturgeon eggs are incubated in MacDonald jars at 13–14° C to prevent fungal infections. Mass hatching takes place 7 days after fertilization and hatching rate can be predicted as a function of the percentage of eggs fertilized. Survival at the end of the endogenous feeding stage is correlated with hatching rate. Egg size has no direct implications for larval growth and survival of Siberian sturgeon. Experimental studies have demonstrated that behavioural observations are useful criteria to assess the quality of larvae and to synchronize the physiological state of fish with the appropriate rearing procedures. Special attention should be given to the transition to exogenous feeding, where cannibalism, difficulties in adaptation to a new diet, overfeeding and resulting bacterial infections dramatically reduces survival to the fingerling stage. Although a commercial artificial diet specifically formulated for larvae of Siberian sturgeon and other acipenserids is still lacking, commercial non-purified rainbow trout diets and starter marine fish diets are currently used and their results are reasonably acceptable in terms of larval growth and survival. Further research must be focused on the determination of egg quality indicators in order to provide the producer with the tools to estimate the viability and performance of theprogeny. 相似文献
45.
Esteban Avigliano Mathieu Leisen Camille Duquenoy Jorge Liotta Alejandra Volpedo 《Austral ecology》2023,48(6):1121-1131
Sturgeon (Acipenser baerii and A. gueldenstaedtii) specimens, native to the northern hemisphere, were reported in different environments of the La Plata Basin (South America). The objectives of this study were to provide the first insights into the natal origin and habitat use of these sturgeon species in the La Plata Basin through geochemical analysis (87Sr/86Sr) of fin spines and to review historical catch reports. Spine core-to-edge 87Sr/86Sr ratios were measured by LA-MC-ICPMS. A Quadratic Discriminant Analysis model based on water 87Sr/86Sr baseline of the La Plata Basin was run to infer the natal origin. The isotopic profiles indicate a common origin, compatible with the location of the fish farms in the Uruguay Basin. The A. baerii isotopic time series suggested that the fish moved towards the Paraná Basin months before capture, while A. gueldenstaedtii would have survived a longer time (perhaps years). Seventeen reports of sturgeons were recorded and preserved in museum collections between 1998 and 2020. Sturgeons were collected from Río de la Plata Estuary, Paraná and Uruguay basins and Atlantic coastal lagoons. It is recommended to closely monitor sturgeon catches, paying special attention to the appearance of specimens of reproductive age, in order to generate management and management plans if necessary. 相似文献
46.
中华鲟受精细胞学研究 总被引:12,自引:2,他引:10
中华鲟(Acipenser sinensis Gray)的成熟卵具有一层放射膜及二层卵黄膜。在动物极有9—15个受精孔。每一受精孔有一大的入口(12.7—13.9μm直径)及一细长的受精管道(1.2—1.3μm直径)。 进入受精孔的许多精子只能按序入卵。其中只有一个精子的头部膨大核化,最后形成雄性原核。同时活跃的卵子也形成雌性原核。雌、雄原核彼此接触,最后融合成合子核,随后分成两个子核。 中华鲟的受精方式为多精入卵,单精受精。 相似文献
47.
Experimental studies indicated that myomeres play several functional roles during swimming. Some of the functions in question are thought to change rostrocaudally, e.g., anterior myomeres are thought to generate forces, whereas posterior myomeres are thought to transmit forces. In order to determine whether these putative functions are reflected in myoseptal morphology we carried out an analysis of the myoseptal system that includes epaxial and hypaxial myosepta of all body regions for the first time. We combined clearing and staining, microdissections, polarized light microscopy, SEM technique, and length measurements of myoseptal parts to reveal the spatial arrangement, collagen fiber architecture, and rostrocaudal gradients of myosepta. We included representatives of the four basal actinopterygian clades to evaluate our findings in an evolutionary and in a functional context. Our comparison revealed a set of actinopterygian groundplan features. This includes a set of specifically arranged myoseptal tendons (epineural, epipleural, lateral, and myorhabdoid tendons) in all epaxial and postanal hypaxial myosepta. Only preanal hypaxial myosepta lack tendons and exclusively consist of mediolateral fibers. Laterally, myosepta generally align with the helically wound fibers of the dermis in order not to limit the body's maximum curvature. Medially, the relationship of myosepta to vertebrae clearly differs from a 1:1 relationship: a myoseptum attaches to the anterior margin of a vertebra, turns caudally, and traverses at least three vertebrae in an almost horizontal orientation in all body regions. By this arrangement, horizontal multiple layers of myosepta are formed along the trunk dorsal and ventral to the horizontal septum. Due to their reinforcement by epineural or epipleural tendons, these multiple layers are hypothesized to resist the radial expansion of underlying muscle fibers and thus contribute to modulation of body stiffness. Rostrocaudally, a dorsoventral symmetry of epaxial and hypaxial myosepta in terms of spatial arrangement and collagen fiber architecture is gradually developed towards the postanal region. Furthermore, the rostrocaudal extension of myosepta measured between anterior and posterior cones gradually increases. This myoseptal region is reinforced by longitudinal fibers of lateral tendons. Furthermore, the percentage of connective tissue in a cross section increases. These morphological data indicate that posterior myosepta are equipped for multisegmental force transmission towards the caudal fin. Anteriormost myosepta have reinforced and elongated dorsal posterior cones. They are adequately designed to transmit epaxial muscular forces to the neurocranium in order to cause its elevation during suction feeding. 相似文献
48.
A Theoretical Study of River Fragmentation by Dams and its Effects on White Sturgeon Populations 总被引:3,自引:2,他引:1
Henriette I. Jager James A. Chandler Kenneth B. Lepla Webb Van Winkle 《Environmental Biology of Fishes》2001,60(4):347-361
Most of the world's large rivers are fragmented by dams. Fragmentation of the river ecosystem alters migration patterns among fish populations and converts free-flowing river to reservoir habitat. In this study, we used an individual-based genetic metapopulation model to study the effects of fragmentation on the population viability and genetic diversity of a large-river fish, the white sturgeon, Acipenser transmontanus. In the first of two simulation experiments, we fragmented a 200km river reach by building 1 to 20 virtual dams. Increased fragmentation produced an exponential decline in the likelihood of persistence, but no extinction threshold to suggest a minimum viable length of river. Compounding isolation with the loss of free-flowing habitat did not further reduce viability until free-flowing habitat was nearly eliminated, at which point extinction was certain. Genetic diversity within (among) populations decreased (increased) as we built the first several dams. Adding more dams caused the number of persisting populations to decline and eroded genetic diversity within and among populations. Our second simulation experiment evaluated the effects of different levels of upstream and downstream migration between river segments. The results of these migration experiments highlighted the importance of balanced migration rates. We found that extinction risk was high for populations linked by high downstream, and low upstream, migration rates, as is often the case in impounded rivers. Our results support the view that migration patterns will play a significant role in determining the viability of riverine fishes, such as the white sturgeon, in river ecosystems fragmented by dams.(retired) 相似文献
49.
Growth and energy budget were measured for three sizes (2.4, 11.1 and 22.5 g) of juvenile white sturgeon Acipenser transmontanus held at 18.5° C and fed tubificid worms at different levels ranging from starvation to ad libitum . For each size-class, specific growth rate increased linearly with increasing ration, and conversion efficiency was highest at the maximum ration. Growth rate decreased with increasing fish size at the maximum ration, but increased with size at each restricted ration. Conversion efficiency increased with increasing ration for each size-class and was usually highest at the maximum ration. Faecal production accounted for 3.2–5.2% of food energy. The proportion of food energy lost in nitrogenous excretion decreased with increasing ration. With increases in ration, the allocation of metabolizable energy to metabolism decreased, while that to growth increased. Fish size had no significant effect on the allocation of metabolizable energy to metabolism or growth. At the maximum ration, on average 64.9% of metabolizable energy was spent on metabolism, and 35.1% on growth. 相似文献
50.
North American Green and European Atlantic Sturgeon: Comparisons of Life Histories and Human Impacts
Joern Gessner Joel P. Van Eenennaam Serge I. Doroshov 《Environmental Biology of Fishes》2007,79(3-4):397-411
Green and European Atlantic sturgeon are listed as a vulnerable and a critically endangered species, respectively. These anadromous
species inhabit different continents but have many similar life history traits and demographic characteristics, including
wide geographic ranges, similar migratory and foraging behavior, age and size structures of reproductive stocks and, historically,
diverse population structures. The differences are limited to tetraploid genome and much larger egg size and lower fecundity
in green sturgeon, reflecting the adaptations to different geomorphology and biota of the Pacific region. Both species have
been affected by over-harvest and habitat losses but the severity of these impacts have been greater and lasted longer for
the European Atlantic sturgeon, resulting in loss of diversity and extirpation of all but one stock. From the comparison of
human impact on two species we conclude that preventive actions should be taken at the early warning signs of changes in population
and recruitment. These should mitigate multiple factors of human activity affecting sturgeon stocks and their habitats. 相似文献